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KMID : 0923620100100050164
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Immune Network
2010 Volume.10 No. 5 p.164 ~ p.172
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Association of Intracellular TH1-TH2 Balance in CD4＋ T-cells and MIP-1? in CD8＋ T-cells with Disease Severity in Adults with Dengue
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Kadhiravan Tamilarasu
Saxena Ankit
Singh Amar
Broor Shobha
Sharma Surendra-K.
Mitra Dipendra-K
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Abstract
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Background: We tested the hypothesis that dengue haemorrhagic fever (DHF) is associated with a TH1-skewed immune response as opposed to dengue fever (DF).
Methods: We estimated intracellular (in T-cells) and serum levels of designate TH1/TH2 cytokines [interferon-? (IFN-?), interleukin-4 (IL-4), and tumor necrosis factor-?] and macrophage inflammatory protein-1? (MIP-1?) at admission, 48 h, and day 5 in 20 adults with dengue (DF=10, DHF=10) and 10 dengue-naive healthy controls.
Results: At admission, intracellular IFN-?/IL-4 ratio in CD4＋ T-cells and proportion of MIP-1?-positive CD8＋ T-cells were significantly higher in patients with DHF [7.21 (5.36∼10.81) vs. 3.04 (1.75∼4.02); p=0.011 and 6.2% (3.2∼8.2%) vs. 2.4% (2.0∼3.6%); p=0.023]. The latter showed a significant positive correlation with IFN-?/IL-4 ratio in CD4＋ T-cells (Spearman’s rho=0.64; p=0.003), percentage-change in haematocrit (rho=0.47; p=0.048), and serum alanine aminotransferase level (rho=0.61; p=0.009).
Conclusion: We conclude that DHF is associated with a TH1-skewed immune response. Further, MIP-1? in CD8＋ T-cells is an important immunologic correlate of disease severity in dengue.
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KEYWORD
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Dengue haemorrhagic fever, Disease severity, Interferon-?, Interleukin-4, Macrophage inflammatory protein-1?
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